cort corticosterone elisa kit  (Reed Biotech Ltd)

Journal: Translational Psychiatry

Article Title: Long-term social isolation during adolescence exacerbated cardiac dysfunction after myocardial infarction

doi: 10.1038/s41398-026-03959-x

Figure Lengend Snippet: A Representative TTC staining images of heart sections in different treatment groups at day 3 post-MI. Scale bar = 2 mm. B Quantification of infarct size defined as the percent of left ventricular area calculated by Image J. C Representative Masson trichrome staining images of heart sections in different treatment groups at day 28 post-MI. The collagen fibers are blue, the muscle fibers are red, and the nuclei are blue-black. Scale bar = 1 mm. D Col1a1 and ( E ) Col3a1 mRNA expression as indicators of myocardial fibrosis were detected by RT-qPCR at day 28 post-MI. F IL-1β, ( G ) IL-18, and ( H ) TNF-α mRNA expression were detected by RT-qPCR at day 28 post-MI. I Representative Western blots images. Quantification of fold changes in protein levels of ( J ) IL-1β, ( K ) IL-18, and ( L ) TNF-α expression were detected by Western blots at day 28 post-MI. M Quantitative comparison of plasma levels of IL-1β determined using ELISA at day 28 post-MI. Data were shown as means ± SEM (Con + Sham group, n = 8; Con + MI group, n = 9-10; SI + Sham group, n = 5; SI + MI group, n = 6). Significance analyzed by two-way ANOVA followed by Sidak ‘s post hoc test. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: All the ELISA kits [NA/NE (Noradrenaline/Norepinephrine) ELISA Kit, E-EL-0047c, Elabscience; QuicKey Pro Mouse CORT (Corticosterone) ELISA Kit, E-OSEL-M0001, Elabscience; Mouse IL-1β ELISA Kit, RK0006, Abclonal] were used and applied according to the manufacturer’s instructions and optical density values were measured by ELISA reader at 450 nm.

Techniques: Staining, Expressing, Quantitative RT-PCR, Western Blot, Comparison, Clinical Proteomics, Enzyme-linked Immunosorbent Assay